Plasmid

Part:BBa_I745006:Design

Designed by: Princeton iGEM2007   Group: iGEM07_Princeton   (2007-10-26)


TRE-Bax-IRES2-EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1488
    Illegal EcoRI site found at 11015
    Illegal XbaI site found at 8889
    Illegal XbaI site found at 18416
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal SpeI site found at 17693
    Illegal SpeI site found at 18367
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10499
    Illegal PstI site found at 12993
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1488
    Illegal EcoRI site found at 11015
    Illegal NheI site found at 1507
    Illegal NheI site found at 11034
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal SpeI site found at 17693
    Illegal SpeI site found at 18367
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10499
    Illegal PstI site found at 12993
    Illegal NotI site found at 85
    Illegal NotI site found at 2865
    Illegal NotI site found at 8876
    Illegal NotI site found at 9612
    Illegal NotI site found at 12392
    Illegal NotI site found at 18403
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1488
    Illegal EcoRI site found at 11015
    Illegal BglII site found at 3630
    Illegal BglII site found at 3696
    Illegal BglII site found at 3737
    Illegal BglII site found at 7929
    Illegal BglII site found at 8934
    Illegal BglII site found at 13157
    Illegal BglII site found at 13223
    Illegal BamHI site found at 1546
    Illegal BamHI site found at 11073
    Illegal XhoI site found at 8883
    Illegal XhoI site found at 18410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1488
    Illegal EcoRI site found at 11015
    Illegal XbaI site found at 8889
    Illegal XbaI site found at 18416
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal SpeI site found at 17693
    Illegal SpeI site found at 18367
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10499
    Illegal PstI site found at 12993
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1488
    Illegal EcoRI site found at 11015
    Illegal XbaI site found at 8889
    Illegal XbaI site found at 18416
    Illegal SpeI site found at 8166
    Illegal SpeI site found at 8840
    Illegal SpeI site found at 17693
    Illegal SpeI site found at 18367
    Illegal PstI site found at 972
    Illegal PstI site found at 3466
    Illegal PstI site found at 10499
    Illegal PstI site found at 12993
    Illegal NgoMIV site found at 4325
    Illegal NgoMIV site found at 5358
    Illegal NgoMIV site found at 5419
    Illegal NgoMIV site found at 5533
    Illegal NgoMIV site found at 13852
    Illegal NgoMIV site found at 14885
    Illegal NgoMIV site found at 14946
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3719
    Illegal BsaI site found at 8916
    Illegal BsaI site found at 13246
    Illegal BsaI site found at 18443
    Illegal BsaI.rc site found at 3479
    Illegal BsaI.rc site found at 7061
    Illegal BsaI.rc site found at 8799
    Illegal BsaI.rc site found at 13006
    Illegal BsaI.rc site found at 16588
    Illegal BsaI.rc site found at 18326
    Illegal SapI site found at 5978
    Illegal SapI site found at 15505
    Illegal SapI.rc site found at 49
    Illegal SapI.rc site found at 9576


Design Notes

We had to make sure there were no SfiI cut sites in the Bax gene.


Source

The iGEM2007 team designed this plasmid.

References